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iMatrix Gel, VM-Gel
Background Information Vistech iMatrix Gel is recombinant Laminin-511-E8 fragments, Laminin-511 is well known to bind to the integrin α6β1 which is located on the cell surface.
Catalog Number VM-Gel
Amount 6 × 175 μg/tube: Solution（PBS），Concentration 0.5 mg/mL, total 6 × 350 μL
Storage and Stability The liquid solution is stable at +2℃-+15℃ until the expiration date printed on the label. Protect from light. Vistech iMatrix Gel is stable at 4℃ for 2 years from the manufacturing date.
Activity The dissociation constant of the binding activity with integrin α6β1 is under 10nM.
Application iMatrix Gel is able to use as cell culture substrate for various cell types including ES/iPS cells.
iMatrix Gel Precoat Procedure 1. Dilute the solution with sterile PBS(-). Coat dishes with 0.5 μg/cm2, Common culture plates and recommended dosages are detailed in the table as follow.
2. Incubate for 1 h at 37℃, 3 h at room temperature, or over night at 4℃. 3. Remove remaining fluid from the coated surface. No rinse is needed. 4. Immediately plate the cells at desired density. * Don’t allow the plate to dry. * Briefly spin down all liquid in the tube before use. * Avoid repeated freeze thaw cycles.
hPSC Passaging Protocol with iMatrix Gel Adding Medium 1. Remove the culture medium. 2. Wash gently with DPBS. Use more than the volume of the spent culture medium. 3. Add the cell dissociation buffer. Incubate at 37°C for 5-7 min. 4. Transfer the cells to a conical tube. Add the culture medium with more than 3 times the volume of dissociation buffer. 5. Centrifuge at 200 g for 3 min. 6. Remove the supernatant fluid. Resuspend gently the cell pellet with appropriate amount of the culture medium. 7. Count the cell number. 8. Seed the cells to new culture plate.
Cell density (recommended) 2×104 cells/cm2，passage every 4 days 1×104 cells/cm2，passage every 5 days
Culture medium:200 μL/cm2
Additives: Y-27632: 10 μM final concentration Vistech iMatrix Gel: 0.25 μg/cm2
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